Volume per well | Validated for 5 to 20 ul |
Detection sensitivity | 1 copy |
Temp uniformity | +- 0.1°C within 1 sec |
Temp stability | +- 0.1°C |
Temperature range | 30 to 100°C |
Average ramp rate | 5.5°C sec |
Thermal system | Hollow silver block peltier-based with conductive fluid |
Block format | 48-well block |
Consumables | 48-well custom plates and optical adhesive seals |
Optical system | Dual LED excitation (452 to 486 nm and 542 to 582 nm) |
Camera | CCD camera |
Filters | 4 emission filters (505 to 545nm 562 to 596nm 604 to 644nm 665 to 705nm) |
Calibrated dyes | SYBR FAM HEX ROX Cy5 |
Additional dyes | All dyes within range compatible with no additional calibration |
Passive reference dyes | Use of ROX is supported but optional |
Data collection | Data always collected in all four filters for all wells |
Plate setup | Plate setup for data analysis can be altered after run completes |
Melt curve | Supports continuous data acquisition in a single filter to provide increased data point collection and reduced run times |
PCR cycle time (unoptimized) | 40 cycles in less than 40 minutes |
PCR cycle time (FAST optimized) | 40 cycles in less than 20 minutes |
Dynamic range | Greater than 9 logs |
Calibration | Not required |
Installation | Plug and play design |
Precision | Discriminates 5000 and 10000 template copies with 99 percent confidence |
Warranty | 1 year (parts and labor included) |
Voltage | 100 to 240 V.AC |
Frequency | 50 and 60 Hz |
Nominal current draw | 5A |
Peak power | 500W (typical power is 180W) |
Software | Multiple license software included at no additional cost |
Chemistry | All chemistries supported |
Applications | Absolute Quantification and Relative Quantification |
Applications | Allelic Discrimination and High Resolution Melt |
Dimensions closed (WxDxH) | 34.5cm x 31cm x 32cm (13.6 in x 12.2 in x 12.6 inches) |
Dimensions open (WxDxH) | 34.5cm x 31cm x 37cm (13.6 in x 12.2 in x 14.5 inches) |
Weight | 13.6 Kg (30 lb) |
CanLab No. | Description | Qty/Uom |
04M0911 | Prime Pro 48 Plates | 50/PK |
14M0911 | Prime Pro 48 Seals | 50/PK |
The Prime Pro 48 real time PCR system is a new, high specification, economically priced real time thermal cycler from Techne. Made in the UK, the Prime Pro 48 features the highest thermal block uniformity and fastest run time of any block-based qPCR system on the market.
High thermal uniformity and validated sample volumes down to 5ul ensures Prime Pro 48 allows researchers to bring run times for an optimised 40 cycle reaction down to only 15 minutes. PCR efficiency relies on precise temperature control during the denaturation and annealing steps. For the highest accuracy, temperature must remain uniform across the plate, ensuring that all samples are processed equally. The unique thermal block design of Prime Pro 48 achieves this with a unique heating and cooling system that quickly cycles from one temperature to the next and then achieves a uniform ±0.1°C in every well of the block within a fraction of a second of reaching each dwell temperature. No other block-based qPCR system can achieve similar performance.
The capacity of Prime Pro 48 accommodates a unique and economical 48-well PCR plate, the size of which is only 1/8 the size of a standard 96-well plate. The well format mirrors a 384-well plate therefore allowing the use of a 16-channel micropipette. The small plate cuts reagent costs in half whilst still producing a strong fluorescence signal. Minimizing the plate size also significantly improves thermal uniformity.
Key Benefits
Speed
Confidence
Performance
Value
Sensitivity